Coordinated regulation of IL-4 and IL-13 expression in human T cells: 3C analysis for DNA looping.

TitleCoordinated regulation of IL-4 and IL-13 expression in human T cells: 3C analysis for DNA looping.
Publication TypeJournal Article
Year of Publication2012
AuthorsYao X, Zha W, Song W, He H, Huang M, Jazrawi E, Lavender P, Barnes PJ, Adcock IM, Durham AL
JournalBiochem Biophys Res Commun
Volume417
Issue3
Pagination996-1001
Date Published2012 Jan 20
ISSN1090-2104
KeywordsAntigens, CD28, Asthma, Base Sequence, Cell Line, Cell Nucleus, Chromatin, DNA, GATA3 Transcription Factor, Gene Expression Regulation, Humans, Immunoprecipitation, Interleukin-13, Interleukin-4, Lymphocyte Activation, Molecular Sequence Data, NFATC Transcription Factors, Nucleic Acid Conformation, Receptors, IgE, Th2 Cells
Abstract

Asthma is a chronic allergic disorder characterised by chronic inflammation. The balance of type I and type II (CD4+) T helper cells is of critical importance. In asthma there is an overexpression of T(H)2 cytokines, such as IL-4, IL-5 and IL-13. The genes encoding these cytokines are located together the same chromosomal region, 5q31.1 in humans. Here we confirm a central role for the transcription factors NFAT and GATA3 in the regulation of human IL-4 and IL-13. Chromatin Conformation Capture (3C) demonstrated the formation of specific ligation products containing spliced IL-4 and IL-13 DNA sequences in human T(H)2 polarised HuT-78 cells. This suggests that co-ordinate expression of T(H)2 cytokines, under the control of GATA3 and NFAT1 is due to the formation of a chromatin hub by DNA looping.

DOI10.1016/j.bbrc.2011.12.069
Alternate JournalBiochem. Biophys. Res. Commun.
PubMed ID22226971
Grant List / / Medical Research Council / United Kingdom

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